Dr. K. D. John MartinLAIJU M. PHILIP2024-05-072024-05-072022-05-12https://krishikosh.egranth.ac.in/handle/1/5810208778A prevalence of clinical lameness of 26.31 per cent was obtained on screening a herd of 114 lactating cows using claw health indicators and infrared thermography during July 2020 and June 2021. Eighteen clinically lame cowsmaintained under identical management conditions with sole ulcer or whiteline disease in one of the hind lateral claws were selected and randomly assigned to treatment into three groups. A positively controlled, randomised clinical trial was conducted for the evaluation of healing of sole lesions with the therapeuticapplication of platelet rich plasma in two different protocols in two groups, against the conventional treatment methods in another group. Autologous PRP was prepared by a modified double centrifugation syringe method in the former two groups of animals, assessed the quality and standardised to 1× 109 platelets/ mL for therapeutic application. Sole lesions in all the animals were treated by therapeutic hoof trimming followed by hoof block application on contralateral claw, dressing with 10 per cent Zinc oxide ointment and hoof bandaging under suitable anaesthetic method. In one group, PRP gel was applied topically over the lesion and in other group, 10 mL of PRP was administered by regional intravenous injection and both treatments were repeated after 48 hours. A remarkable improvement in the clinical healing and wound closure were observed in PRP IVRA group than PRP gel and control groups in the early phase of healing. PRP IVRA group had better quality horn and less pain sensitivity. The greater expressions of IL1α, IL1β, IL10, Hp and MMP13 genes were observed in lame cows compared to sound cows. Relative gene expression of cytokine was downregulated by IVRA of PRP progressively during the healing of sole lesion at all the time points. Histopathological examination and post therapy locomotion score revealed no significant differences between the treatment groups at any observation time. Hence the present study concluded that intravenous regional infusion of PRP could be considered as better regenerative therapy along with therapeutic hoof trimming for the management of sole ulcer and whiteline disease in dairy cattleEnglishThesis