KULDEEP SINGH DANGIHARI KISHAN, S2016-08-262016-08-262004http://krishikosh.egranth.ac.in/handle/1/73757The investigations were carried out to evaluate five new diverse CMS sources for stability of cytoplasmic male sterility, per se performance, physiological and biochemical characterisation of CMS lines. The material comprised of 22 genotypes which included 10 CMS lines from five diverse cytosterile sources viz., PET-1, PET-2, GIG-1, CMS PF and CMS-I, their maintainers and two standard checks (CMS 7-1A, CMS-234A). The experiments were conducted at Directorate of Oilseeds Research Farm, Rajendranagar, Hyderabad during kharif, 2000 and rabi, 2000-01. The analysis of variance revealed that significant variability was present in all the genotypes. Among the five diverse CMS sources, CMS PET-2 recorded maximum mean performance for most of the characters i.e., days to 50 per cent flowering, days to maturity, head diameter, number of filled seeds per head, number of leaves per plant, oil percentage, oil yield per plant and seed yield per plant in kharif as well as in rabi seasons. The stability studies indicated that CMS-I and CMS-PF were unstable whereas, PET-1, PET-2 and GIG-1 sources were stable in all the sowing dates throughout the year. Hence, the cytosterile sources, PET-1, PET-2 and GIG-1 could be utilised in hybrid development programme. The study of physiological parameters revealed that maximum leaf area index of 3.81 was obtained in CMS lines taken from GIG-1 cytoplasm followed by CMS-I and PET-2 cytoplasm. Maximum LAI was found at the time of 60 DAS and later it declined. In case of total drymatter production and harvest index, PET-2 source of cytoplasm showed the highest values. The data on fatty acid composition of sunflower oil revealed that the total unsaturated fatty acids (oleic and linoleic acids) were higher than the total saturated fatty acids (stearic and palmitic acids). The CMS lines from PET-1, PET-2 and GIG-1 cytoplasms recorded highest linoleic acid and oleic acid content. These lines with high amounts of oleic and linoleic acid may be used in hybridization programme for developing new hybrids as high amounts of these two essential fatty acids is of considerable significance with respect to both quality and human diet. Based on the present study it can be concluded that, PET-1, PET-2 and GIG-1 cytoplasms are not only stable but also showed high amount of total unsaturated fatty acids composition and good per se performance for all the characters studiedenIDENTIFICATION, STABLE, CYTOPLASMIC, MALE STERILE, LINES, SUNFLOWERThesis