Dr. P. H. VataliyaPATIL AJAY VITTHAL2017-07-272017-07-272008http://krishikosh.egranth.ac.in/handle/1/5810026387India has emerged today as the largest producer of milk in the world crossing 97 million tones annually. The organized dairy sector in India is largely dependent on buffalo milk as they contribute about 48 percent of the total milk produced in the country. Molecular genetic technologies will aid conventional methods of increasing production potential of indigenous buffaloes by augmenting genetic gain in a relatively shorter span of time. The candidate gene approach provides tools to search for causative polymorphisms affecting quantitative traits. Signal transducers and activators of transcription (STAT5A) and peroxisome peoliferator activated receptor gamma cofactor 1 alpha (PPARGC1A) are transcription factor encoding genes that are proved to be having candidate roles in milk production. The present work was carried out to study the polymorphism in STAT5A exon 7 and exon 10 and PPARGC1A partial exon 9 and intron 9 gene fragments by PCR-SSCP, PCR-RFLP and sequencing in Mehsana buffaloes.enAnimal BiotechnologyAnalysisThesis