Singh, NageswerReetranjan2019-01-312019-01-312017-07-18http://krishikosh.egranth.ac.in/handle/1/5810093114In the present investigation entitled “Phytochemical properties, interaction and expression analysis of Glyceraldehyde-3- phosphate dehydrogenase (GAPDH) in Cucumis sativus infected with Cucumber mosaic virus” is focused on the effect of Cucumber mosaic virus(CMV) on phytochemical constituents in the leaf samples of three cultivars of C.sativusviz.Summer Green, Green Star and Green Long along with interaction and expression analysis of different forms of GAPDH. Phytochemical properties viz. total phenols; antioxidant activity and PPO activity have higher values in infected leaf samples whereas total chlorophyll and carotenoids had lower values in infected leaves. The inoculated cultivars had lower potassium content; however sodium content had no uniform trend in the infected leaf samples.Protein- protein interaction analysis by yeast two hybrid system revealed that GAP C is one of the host proteins which interacts with the movement protein of CMV thereby,one of the reason to be playing role in virus movement from one cell to another.Expression studies of GAPDH forms (GAP A; GAP B and GAP C) in CMV infected cultivars varied at 0,3,5,7 and 14 dpi andit was in accordance with the resistant and susceptible nature of the cultivar. The plant is either using its defense mechanism or the host proteins are being utilized by the virus for its propagation through upregulation or downregulation of the GAPDH as observed in expression analysis investigation.ennullThesis