Jakhar, B.L.Jat, Hansa Kumari2024-04-032024-04-032022https://krishikosh.egranth.ac.in/handle/1/5810208068Dissipation and Persistence Kinetics of Fipronil and flubendiamide on Cabbage and Monitoring of insecticide Residues in Vegetables was studied, following the application at recommended dose (40 and 18.24 g a. i. ha-1 ), respectively and double of the recommended dose (80 and 36.48 g a. i. ha-1 ), respectively, in the Division of Entomology, Pesticide Residue Laboratory, Rajasthan Agricultural Research Institute, Durgapura, Jaipur (Rajasthan) during (2020-21). The persistence study on cabbage recorded the average initial deposit of pesticide fipronil, its three metabolites desulfinyl (MB046513), sulfide (MB045950) and sulfone (MB046136) were 0.641, 0.377, 0.065, and 0.327 respectively and flubendiamide on cabbage was recorded as 0.292 mg kg-1 at recommended dose, while at double of the recommended dose, the average initial deposit were 1.077, 0.737, 0.115, and 0.677 for fipronil and its metabolites and 0.506 mg kg-1 for flubendiamide. The control sample of the cabbage show the residue of the fipronil, its three metabolites and flubendiamide, respectively. The residues half-life (RL50) values were 2.4, 2.7, 2.7, and 2.6 days for fipronil and its metabolites at recommended dose and 3.6 days for flubendiamide while at double of the recommended doses, it is 4.3, 2.6, 3.6, 2.9 and 4.5 for respectively. The waiting periods for the respective pesticides were worked out at their limit of determination (0.001 and 0.05 mg kg-1 for fipronil, its metabolites and flubendiamide) due to lack of MRL value by the FSSAI. In case of soil samples the residues at harvest time of cabbage crop was not detected in the recommended dose and double of the recommended dose, respectively. In decontamination studies, acetic acid treatment was significantly superior to rest of the treatments followed by lukewarm water, NaHCO3 5 percent, NaCl 1 percent NaCl, KMnO4 and tap water washing were recorded in reduction of fipronil and its metabolites residues. While in reduction of flubendiamide residues, lukewarm water treatment was significantly superior to rest of the treatments followed by acetic acid 5 percent, NaHCO3 5 percent , NaCl 1 percent, KMnO4 and tap water washing. Out of 40 samples of different farmgate vegetables analysed, 10 vegetable samples (25%) were detected with different pesticides residues. While, out of 40 market vegetables samples analysed, 12 vegetable samples (30%) were found contaminated with different pesticide residues. None of the samples showed pesticide residues exceeding the MRL in farmgate and market samples. Therefore, the study concluded that fipronil was the most persistent followed by desulfinyl, sulfone, sulfide and flubendiamide as least persistent among test pesticides on cabbage. The safe waiting period on cabbage were suggested as 5, 6, 3, 6 and 6 days at recommended dose and 10, 7, 7, 7 and 8 days at double of the recommended dose for fipronil, its metabolites and flubendiamide, respectively. In case of fipronil and its metabolites, acetic acid was found as the most effective decontamination process. While in case of flubendiamide luke warm water was significantly superior to rest of the treatments. Pesticide monitoring study is a continuous one and the real dimension of the problem may be apprehended only after widespread monitoring with adequately large sample sizeEnglishThesis