Saharan, Hawa SinghPreeti2022-09-192022-09-192020https://krishikosh.egranth.ac.in/handle/1/5810187846The charcoal rot disease caused by Macrophomina phaseolina (Tassi) Goid, is the most destructive soil and seed borne disease of sesame (Sesamum indicum L.) which appears every year in Haryana and causes heavy losses in yield. Hence, the present investigation was undertaken to study the eco-friendly management of M. phaseolina through botanicals and bio-agents under in vitro and in vivo conditions in screen house of Department of Plant Pathology, CCSHAU, Hisar. Among the botanicals evaluated for their efficacy in vitro against M. phaseolina, the phytoextract of Lantana camara inhibited maximum mycelial growth by 89.43 per cent at 20 per cent concentration followed by Parthenium hysterophorus and garlic (Allium sativum) extracts which inhibited upto 87.21 and 57.21 per cent, respectively at 20 per cent concentration. However, ginger (Zingiber officinale) was found to be least effective in inhibiting of mycelial growth by 26.94% at 20% concentration. Evaluation of bio-agents for their efficacy against M. phaseolina in vitro revealed that Trichoderma harzianum showed maximum antifungal activity with 66.94, 59.99, 59.44 per cent of mycelial inhibition by dual culture technique, production of non-volatile and volatile compounds techniques respectively, which was followed by T. viride with 59.16, 55.27, 53.00 per cent mycelial inhibition, respectively. Pseuodomonas fluorescens was found to be least effective with 30.83, 51.38, 47.77 per cent mycelial inhibition by dual culture technique, production of non-volatile and volatile compounds techniques respectively. Among the combinations of phytoextracts and bioagents tested under screen house conditions, seed soaking in solution of 20 per cent concentration of L. camara extract for 5-10 minutes followed by seed treatment with T. harzianum @ 10g/kg seed was found most effective in controlling the disease upto 36.43 per cent in HT-1 and 40.92 per cent in HT-2 varieties followed by combination of P. hysterophorus + T. harzianum which controlled the disease up to 34.28 and 38.53 per cent in HT-1 and HT-2 varieties, respectively.EnglishThesis