Dr. D. J. GhodasaraPatel Nikul. N2017-05-042017-05-042011http://krishikosh.egranth.ac.in/handle/1/5810010562The present research work was conducted on 24 male and 24 female Wistar rats to study the toxicopathological effects of repeated dose (28 days) of methotrexate. Wistar rats were randomly divided into 4 different groups with six males and six females in each group. Animals of group II to IV were given 0.062, 0.125 and 0.250 mg/kg b. wt methotrexate by gavage for 28 days where as group I was administrated only distilled water as (vehicle) control. After completion of 28 days treatment, blood samples were collected for hematology and serum biochemical analysis from retro-orbital plexus with the help of capillary tube. The animals were sacrificed by high dose of anesthesia with Di - ethyl ether. Necropsy examination was performed in all sacrificed animals and gross lesions were recorded. Tissue samples (lung, liver, kidney, intestine, testes and heart) were collected in 10% formalin solution for histopathological examination. The extent and severity of observed symptoms varied according to tenVeterinary PathologyStudiesThesis