RAMAPPA, H K.CHIRAG GAUTAM2023-01-182023-01-182021-10-18https://krishikosh.egranth.ac.in/handle/1/5810192523ThesisMungbean Yellow Mosaic Virus (MYMV) disease of mungbean causes severe losses to crop yield worldwide. Disease incidence ranged between 27.06-87.80 and 29.67-95.58 per cent during Rabi summer 2016-17 and 2017-18 respectively. The highest per cent disease incidence and whitefly population was recorded in Chamarajanagar district. Molecular confirmation of diseased samples was done by using CP specific set of primers. Maximum transmission efficiency (80 %) was observed when ten or more whiteflies per plant were used. Maximum (90%) per cent transmission was observed at an AAP and IAP of 24 h. MYMV isolate of Chamarajanagar, Mandya, GKVK and Tumakuru produced various symptoms when inoculated on susceptible mungbean genotype (BGS-9). Host plants viz., mungbean, urdbean, soybean, cowpea and horsegram noculated with MYMV recorded 30 to 70 per cent disease incidence. However, pigeonpea and dolichos did not develop any symptoms. Cross infectivity of YMV from horsegram and urdbean showed chlorotic and leaf deformation symptoms on mungbean genotype BGS-9. AC1, AC2, AC3, AC4 and AV1 of DNA A and BC1 of DNA-B of Chamarajanagar, GKVK and Mandya isolates were amplified by using gene specific set of primers. BLAST results and phylogenetic analysis revealed that all the genes from different isolates had some variability within but showed similarity with MYMV. Out of 150 mungbean genotypes screened, 10 genotypes found moderately resistant against MYMV. Seed treatment with imidacloprid 48 FS @ 5.0 ml/kg seed+ one spray with imidacloprid 17.8 SL @ 0.24 ml/l (T8) was highly effective in reducing whitefly population by 60.82 and 56.48 per cent, and disease incidence by 56.37 and 50.38 percent, resulted in highest yield (1095.00 and 1132.23 Kg/ha) during 2016-17 and 2017-18, respectively.EnglishThesis