SHARMA, RAJNISHSHARMA, HIMANI2017-05-152017-05-152014http://krishikosh.egranth.ac.in/handle/1/5810011194ABSTRACT The family Rosaceaeis third most economically important plant family that include temperate pome fruit crops having a wide genetic diversity. For a rationale use of the genetic resources in breeding programs it is necessary to understand relationship within and among related crop varieties and to assess their transferability to the species with little genomic information making them significant for genetic research. Thus, in the present study genetic relatedness and SSRs transferability among fifty genotypes of temperate pome fruit crops were tested using (RAPD, ISSR and SSR) molecular marker analysis. The level of polymorphism across genotypes was 97.29% and 97.45% by RAPD and ISSR markers, respectively. The dendrogram derived from RAPD and ISSR analysis grouped temperate pome fruit crops into two major cluster comprising of 40 (cluster A) and 10 (cluster B) genotypes which branched at similarity value of 0.31 and 0.33, respectively. Estimates of pooled similarity coefficient ranged from 0.30 to 0.94. It was concluded from the pooled analysis of both the molecular marker that 50 genotypes of temperate pome fruit crops were grouped in two clusters A and B comprised 45 and 5 genotypes, respectively which branched at similarity value of 0.30 with the maximum similarity of 94% was found between EC-02520 and EC-024530 quince genotypes. Further, out of 13 and 15 screened apple and pear genomic SSR, 10 (76.92%) and 11 (73.33%) produced amplification in 20 apple and pear genotypes, respectively. Highest transferability of apple and pear SSR, 61.53% and 73.33% was observed in closely related quince and apple genotypes, respectively. This high level of polymorphism and good transferability of apple and pear SSRs to other temperate pome fruit crops indicated their promise for application to future molecular screening, map construction, and comparative genomic studies, etc.ennullThesis