Hemavathi, NHayath Basha, SabihaKumary, S. UshaTANUVAS2020-02-282020-02-282020-02http://krishikosh.egranth.ac.in/handle/1/5810143901TNV_IJCMAS_2020_9(2)2929-2934Hair follicle is an ideal system for studying the biology and regulation of adult stem cells. Multipotent stemness of the canine hair follicle stem cells (cHFSCs) were detected by immunostaining the cells with positive HFSC surface markers like Anti-cytokeratin-15 (CK15) and CD200. In this study, canine hair follicular cells were cultured in Williams’E media supplemented with growth factors. The primary cultures of the hair follicles were subjected to Fluorescence Activated Cell Sorting (FACS) and then cells were immunostained with CK15 and DAPI. In primary culture, 18.28% of cells were positive for CK15 and sorted out by FACS. The positive expression of CK15 transcription factor was observed with DAPI as a counter stain in the in-vitro cultured cHFSCs. These results indicated that the cells in the canine hair follicular bulge region possess the stemness and act as a precursor and involved in hair cycle.enVeterinary ScienceArticle