Sharma, AbhishekDhadly, Dalvir Kaur2023-12-112023-12-112023Dhadly, Dalvir Kaur (2023). Characterization of Badnavirus (es) Species associated with Yellow Mosaic Disease of Citrus in Punjab (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.https://krishikosh.egranth.ac.in/handle/1/5810202483Citrus is attacked by a large number of viral diseases worldwide, some being confined to a particular region. Among these Citrus yellow mosaic virus disease (CiYMVD) is restricted to India. Only one Badnavirus species i.e. Citrus yellow mosaic virus (CiYMV) has been reported to be associated with this disease. CiYMV is a double stranded DNA (dsDNA) plant pararetrovirus virus belonging to family Caulimoviridae. In the present study, Citrus growing districts of Punjab i.e. Fazilka, Hoshiarpur and Ludhiana were surveyed and a variety of symptoms were observed on different Citrus species like yellow specks, yellow spots, yellow patches, leaf puckering, leaf mottling, vein clearing and vein banding. Incidence of Citrus yellow mosaic virus disease ranged from 6.7-23.3 per cent in Punjab. For the detection of Badnaviruses, rolling circle amplification (RCA) followed by PCR approach was found reliable. Citrus yellow mosaic virus and Gulupa bacilliform virus A were characterized from Citrus samples L-11 and H-6 collected from Ludhiana and Hoshiarpur respectively. From mealybug samples M5 and M4, Banana streak virus and Sugarcane bacilliform virus were characterized. While Cacao red vein-banding virus was characterized from bael tree growing in the vicinity of Citrus orchard. Badnavirus positive Citrus species were T-budded (i.e. used as scions) onto Rough lemon and Carrizo rootstocks, the virus was successfully transmitted to scion leaves and confirm the occurrence of CiYMVD in Punjab.EnglishThesis