Parthiban, M.Senthilkumar, S.Balakrishnan, G.Aarthi, K.TANUVAS2019-12-272019-12-272015-07http://krishikosh.egranth.ac.in/handle/1/5810138906TNV_IJAS_85(7)_Jul2015_705-708The outer membrane protein, viz. OmpL1 of Leptospira interrogans Icterohaemorrhagiae was cloned into pETl5b vector. The recombinant colonies were expressed in E. coli BL21 DE3 cells. The expressed proteins were checked in SDS—PAGE and western blot analysis. Similarly. the whole cell antigen was prepared using Leptospira interrogans Icterohaemorrhagiae culture.en-USVeterinary ScienceVeterinary PhysiologyArticle