Boora, K.S.Mehta, Deepa2016-11-302016-11-302005http://krishikosh.egranth.ac.in/handle/1/88321Sunflower (Helianthus annuus L.) ranks second among oilseed crops in the world after soybean. Sunflower is protrandrous. Seed set in sunflower is a complex phenomenon and one of the mean to overcome this problem is by identifying self fertile and open pollinating lines. The present study was undertaken to identify molecular marker closely linked to self compatible and self incompatible gene(s) in sunflower using RAPD analysis. A total of 26 genotypes (13 self compatible + 13 self incompatible) of sunflowers were used. Protocols were optimized for DNA extraction and PCR amplification of self compatible and self incompatible genotypes of sunflower using RAPD markers. DNA was isolated using CTAB method with some modifications and among different genotypes, CMS 338 (C)A gave highest quantity (1034.5 μg/ml) of DNA whereas genotypes Acc-1445-6 gave lowest amount (317.5 μg/ml) DNA. Quality of DNA was tested by agorase gel electrophoresis and UV spectrophotometer. A single discrete band of high molecular weight showed that DNA was pure and free from contaminants. Optimum PCR amplification was observed on all DNA samples when reaction mixture contained genomic DNA (100 ng), MgCl2 (1.5 mM), Taq DNA polymerase (3 units), dNTPs (200 M), 10X Taq DNA polymerase buffer (1 μl), primer (10 μM) and annealing temperature of 400C. A total of fifty four random primers were screened and thirty eight primers produced polymorphism. These thirty eight primers were screened with self compatible and self incompatible bulks. Primer OPA-9 and OPE-2 produced a unique DNA band in self incompatible and self compatible bulks, respectively. Marker OPA-9 produced unique band in all the self incompatible genotypes except genotypes IHT-298 and HB-342. This marker is 7.6 cM away from the genes for self incompatible. Similarly, marker OPE-2 produced unique band in all the self compatible genotypes except IB-43, Acc-1351-3, HB-105. This marker is about 11.53 cM away from the genes for self compatibility. Marker OPA-9 produced a unique band in twelve self compatible genotypes. Marker OPE-2 produced unique band in eleven self incompatible genotypes. These markers may be proved useful in marker assisted selection for self compatible and self incompatible traits, respectively.enDNA fingerprinting of self-Compatible and self-Incompatible genotypes of sunflower (Helianthus Annuuns L.)Thesis