Nirmalan, GRamakrishna Pillai, M GKAU2020-10-212020-10-211978170037https://krishikosh.egranth.ac.in/handle/1/5810153510PhDInformation on the specific role of onzymes in controlling the various biochemical events leading to formation of an egg in the avian oviduct is scanty. Hence, it was considered worth while to investigate the onzyme pattern in the plasma and in the reproductive organs in white Leghorn and white Plymouth Rock breeds of fowls, and to studyt the tissue localization of acid and alkaline phosphatases by histochemical techniques. The influences of various oxogenous sex hormones on the development and onzyne pattern of the female reproductive organs was also studied. The blood plasma and tissue homogenatee of the reproductive organs were assayed for alkaline phosphatase, acid phosphatase, glutamate oxaloacetate transaainace , acid phosphatase, glutante pyruvate transaminases and glucose-6- phosphatase. Many of the enzymes studied were present in the plasma and tissue homogenates without any significant breed difference. Acid phosphatase activity could not be detected in he blood [plasma of one-to-two-months-old white Leghorn and White Plymouth rock birds. In the white plymouth rock chicken,ma glutanate pyruvate transaminase activity also could not be detected. Alkaline activity was higher in the ovary of White Leghorn breed of fowls. phosphatase In the two-to-three-month-old pullets, White Plymouth rock bird showed higher concentration of glucose-6-phosphatase in their oviducts. Five-to six-month-old folwle of both the breed did not show any plasma acid phosphatase activity. But in the infundibulue, activities of both the transminaces were higher in the White Leghorn fowle. Glutamate pyruvate tranminance activity in white Plyouth rock oviducvt was conficed to the megaum and isthmue. While there was not significant difference in the morophological development of the oviduet in immature chicken under the influences of different combination of stilbestrol diprepienate and testoateronar propionate or stilbestrol dipropionate and progeetrone compared to chickens on stil bestrol dipropionate alone the White Leghorn chickens receiving stilbostrol dipropinate and testoaterins propicnate showed higher ovariam levels of acid phosphatase and glutamate transminase. pyruvate Oviducal contents of acid phosphatase, glutamate oxalocetate and glutamate pyruvate transminases were also high. In the white Plymouth rock ehicken, on the other hand, lower concentration of plasma glutnate pyruvate transmainase and lower levels of oviducal alkaline phosphatase, acid phophatase and glutanate oxalocetate transminace were evident. In between the two experimental groups, the white Plymouth rock chickens had higher content of acid phosphatase in the iethmus and glucose-6-phosphatase in the varina. But, among the control groups higher contents of plasma glutamae pyruvate transaminase and oviducak alkaline phosphatase were seen in he whit plymouth rock chicks. Stilbestrol dipropionate and progreaterone administation resulted in higher oviducal concentraion of glutamate oxalocacetate tamsmionase in white plymouth rock chicks. Glutamate pyruvate tranmnase activity could tissues studied. In white Leghorn experimental chicks the ovarian acid phosphatase content was less than their corresponding control chicks. Histochemical localisation of alkline phosphatase was seen in all the region of the female reproductive organs in both the breeds. Ovarian localization of the enzyme was in the follicular optithelium and strima with strong reaction for the enzyme in many of the blood veacole. The reaction was of stronger intensity in magmm and gvagina coupared to other regionsof the oviduct. Localization of acid phosphatase was similar to that of alkaline phosphatase. The histochemical localization of acid and alkaline phosphatase in the various regions of the female reproductive organs developed under the influence of different sex hormones were similar to those seen in adult fowls. However, the intensity of the staining reaction for the ensyme in the reproductive organs of chicks developed under the influence of stilbestrol dipropionate was more compared to that of the other two experimental groups.EnglishThesis