PRABHU, G. A.VADODARIA, V. P.2018-04-202018-04-201987http://krishikosh.egranth.ac.in/handle/1/5810044254The study of physico-chemical and bioch^jlcal pi-operties of cervical iaucus was carried out on healthy breeding twffaloes and heifers of Mehsani breed which t were brou^it for artificial insemination during estrus $ period, fhls study was undertaken with mx objective of knowing the Physico-Biocheaiical profile of cervical mucus in these aniaals at fertile insemination. The physical nature of cervical mucus (voluae, colour, consisteacy, ispecific gravity, pH and dry matter) and different physiological conditions of aniaals (lactational status, estrus intensity, estrnxs stage ar«i uterine tone) in relation to conceptive inseminations in tliese animals were investigated, a © results indicated that the significantly greater volume (PL0.01) and lower alkaline pi I (PL0.05) of cervical mucus favoured conceition, A relatively higher conception rate (60.17%) was found in animals with thin cervical mucus than in those with thick taucus (39.83%). More thai 505S of animals at conceived had turbid and opaque cervical mucus Significantly higher levels of Na+(PL0.05) Zn++(PL0.0l),Mn++(PL0.01), Fe++(PL0.0l),glucose(PLO.05), LDH(PL0.01) and sisaifieantly lower levels of K+(PL0.05), C 1-( PL0.01 ) , Ca++(PL0.01 ), Mg++( PL0.01 ), Cu++( PL0.05), glycogen(PL0.01),TC (PL0.01),TP (PLO.Ol), SA (PL0.01) and PBH (PL0.01) in cervical mucus favoured the phenomenon of coception, These differences indicated their use in, tentatively, predicting the fertility status in buffaloes and heifers of Mehsani breed. Na+ levels in clear, turbid and opaque cervical mucus differed significantly. Its highest (PLO.01) and lowest (PL0.01) levels were recorded in opaque and clear mucus respectively. K+ value during late heat was lowest and it differed significantly (PL0.01) from the values recorded during early and mid heat. Cu++ content of buffloe's cervical mucus was lower (PL 0.01) than that of heifers, as regards to estrus stage and uterine tone. Sialic acid increased (PL 0.05) with increase in estrus intensity. However, its level in cervical mucus remained lowest (PL0.05) in buffaloes than that in heifers. Both fucose and protein bound hexose values in cervical muscus remained lowest(PLO.O5) during first lactation when compared with their values in other eight comparative levels . Their highest (P 0.05) values were recorded in cervical mucus of non-lactating non-conceived buffaloes. Overall alkaline phosphatase activity of cervical muscus was found to be lower (PL.0.01) in buffaloes than heifers . Acid phosphatase remained, lower (PL0.01) in conceived group compared to non-conceived group, when studied under lactational status. Similarly, it was higher (PLO.05) in conceived group during weaker uterine tone. On an average buffaloes recorded higher (PL0.05) ACP activity than heifers. LDH activity was recorded higher (PL0.01) in conceived group than non-conceived group, when cervical mucus was tested for consistesicy.enANIMAL PHYSIOLOGYA STUDYThesis