B. FakrudinAbhijit Daspute2016-11-182016-11-182012http://krishikosh.egranth.ac.in/handle/1/86336Pigeonpea Sterility Mosaic Disease (PSMD) caused by Pigeonpea Sterility Mosaic Virus (PPSMV) and transmitted by Eriophyid mite (Aceria cajani Channabasavanna) is an important disease of pigeonpea. Inheritance of PSMD resistance was studies in two crosses: in Gullyal white x BSMR 736 cross, out of 325 F2:3 families field evaluated, 55 were resistant and 270 were susceptible, while, in ICP 8863 x BSMR 736 cross, out of 225 F2:3 families field evaluated, 38 were resistant and 187 were susceptible to PPSMV with segregation ratios (susceptible: resistant) of 270:55 (P 0.05) and 187:37 (P 0.05) respectively, which is comparable to 13 (susceptible):3 (resistant) ratio. Hence, inheritance of PSMD resistance was found to be controlled by two genes, SV1 and SV2, with inhibitory gene interaction both the crosses. The parents of the crosses were tested with 300 RAPD and 20 SSRs markers and in all 95 and 3 amplicons respectively recorded polymorphism. Bulked Segregant Analysis (BSA) revealed IABTPPAK-19, IANTPPN-18 and IABTPPL-2 RAPD markers association with susceptibility and IABTPPN-7 association with both resistance and susceptibility in coupling-repulsion: these markers segregated in expected 3:1 ration in F2 individuals of both crosses, while SSR marker, CcM0844 in 1:2:1 ratio. Single Marker Analysis (SMA) recorded 15, 8, 22, 20, 36, 5 and 3 per cent phenotypic variance respectively for IABTPPAK-191295, IABTPPN-18827, IABTPPL- 2898, IABTPPN-7983, IABTPPN-7414 and CcM0844 markers. Association of these markers with PSMD resistance/susceptibility was validated in 12 genotypes of known reaction to PSMD. Linked RAPD markers were sequence characterised and prospected for Sequence Characterised Amplicon Regions (SCAR) markers; one marker, FlnthN- 18, was found co-segregating in Gullyal white x BSMR 736 cross in 3:1 ratio. Single Nucleotide Polymorphisms (SNPs) were identified in three out of five linked RAPD amplicons. Further, field evaluation of lines from both crosses lead to at a subset which is resistance to PSMD with a potential breeding value.Thesis