Huozha, R.Thakur, Swati2020-11-202020-11-202020-10https://krishikosh.egranth.ac.in/handle/1/5810155412Badri cattle is first registered indigenous cattle breed of Uttarakhand, mainly reared in hilly areas of Kumaon and Garhwal regions. It is a dual purpose breed, well adapted to hilly areas, prevailing climatic conditions and resistant to many diseases. This breed has poor milk yield (0.5 to 2.0 kg/day) with short lactation length (208 ± 20 days), but have excellent drought ability and requires less external inputs. Several factors that affects the normal physiology, out of which lactation and pregnancy period are highly affected. Total forty Badri cows (200±50 kg BW, 1-6th parity) belonging to different lactation stages were selected from the conservation unit maintain at IDF, Nagla GBPUAT, Pantnagar for the present study to investigate their hemato-biochemical entities, osmotic fragility test of erythrocytes and milk somatic cell count. Selected cows were divided into five groups according to their lactation stages as early, early mid, late mid, late lactation stages and another group of non-lactation having eight cows in each group. Both blood samples and milk samples were collected. Hematological entities demonstrated a reverse pattern of values between PCV and ESR during lactation stages. TEC was lowest in late mid lactation while appear highest during early mid and late lactation stages. MCV and MCH were highest during late mid lactation and lowest during early and early mid lactation stages. DLC lymphocytes was highest during early lactation stage. Percent hemolysis of erythrocytes was highest during early mid lactation at 0.9% and 0.5% saline concentration. Serum total protein and albumin were highest during early lactation and decreased as advance in lactation stages while serum creatinine, glucose, cholesterol and triglycerides showed reversed pattern. Serum ALT was highest in early mid lactation and lowest during early and late mid lactation stages. During late lactation serum ALP was highest whereas CK was lowest and during late mid lactation serum ALP was lowest whereas CK was highest. Serum phosphorous during late mid lactation showed lowest while Ca: P ratio was highest. Milk somatic cell count (SCC) was increased during early lactation, late mid lactation and decreased during early mid and late stages of lactation. Thus, concluded that different stages of lactation highly affected the normal physiological state which imposed an alteration in the metabolism of lactating cows.EnglishThesis