Leela WatiRekha2018-04-272018-04-272018http://krishikosh.egranth.ac.in/handle/1/5810044644Mungbean (Vigna radiata (L.) Wilczek), also known as green gram, is one of the important pulse crop of India. India alone accounts for 65% of its world acreage and 54% of the production. Nonrhizobial endophytic bacteria exert several beneficial effects on host plant such as stimulation of plant growth, nitrogen fixation and induction of resistance to plant pathogen. The use of endophytes offers an attractive way to replace chemical fertilizers, pesticides and supplements. In the present study, Mungbean nodule samples were collected from farm area of Pulses section, Genetics and Plant Breeding, CCS HAU, Hisar during summer and kharif season to isolate the endophytes. In total, 41 endophytes were isolated using tryptone soya agar (TSA) medium. After isolation, all the endophytes were assessed for temperature tolerance (up to 450C) and salt (NaCl) tolerance (up to 5%). The majority of mungbean endophytes were found to be IAA producers, though the production varied in the range of 1.12-44.88 μg/ml and the P-solubilisation activity of endophytic isolates on solid as well as liquid medium was restricted to 49 % of the isolates only. Ammonia excretion varied in the range of 0.25-3.47 μg/ml and isolate E17 exhibited maximum ammonia excretion (3.47μg/ml). Out of 41 isolates only 10 endophytic isolates were siderophore producers and 12 endophytic isolates showed HCN production and overall 76% of mungbean nodule endophytic isolates showed ACC utilization on minimal medium plates. Potassium solubilisation was shown by only 24% of the endophytic isolates. Four promising endophytic isolates, namely E6, E13, E14 and E17 which showed maximum number of plant growth promoting traits were selected for plant growth evaluation of mungbean under pot house conditions during summer and kharif season (2016). Among various treatments, coinoculation of endophytic isolate E13 along with Rhizobium (MB703) and phosphate solubiliser (P36) was the best treatment with respect to nodulation, plant growth and yield attributes under pot house as well as field conditions. On the basis of biochemical characterization and partial 16S rDNA sequencing isolates E6, E13, E14 and E17 were identified to be Pseudomonas sp. strain PM1220, Bacillus amyloliquefaciens strain PM1218, Bacillus flexus starin PM1217and Beijerinckia fluminensis strain PM1219.ennullThesis