KanikaRAKESH BHOWMICK2016-09-232016-09-232012http://krishikosh.egranth.ac.in/handle/1/78685T-8602Present study was conducted to screen the genes induced by salinity stress at two different growth stages (seedling and tillering) in Kharchia Local wheat. Physiological analysis was carried out in both salt tolerant Kharchia local and salt susceptible HD2009. The study revealed that Kharchia local maintained better physiological state as compared to HD2009 during salinity stress. Subtractive cDNA libraries were constructed from two different developmental stages (seedling and tillering stages). Two hundred and eighty eight recombinant clones were sequenced from each library. Sixteen percent of the genes identified in seedling stage and twenty five percent in tillering stage were related to stress. These included catalase, superoxide dismutase, glutathion reductase, WRKY 80 transcription factors, aldehyde dehydrogenase etc. Some genes like translationally controlled tumor protein, poly A binding protein, senescence associated proteins were also induced by salinity treatment. More than 25% of the ESTs generated fell in unknown/no hit category upon homology search through BLAST analysis. Differential expression of three genes was confirmed by RT PCR analysis. Key Words: Suppression Subtractive Hybridizatioplanting, livestock, rice, biological phenomena, productivity, group communication, crops, diseases, yields, economic systemsThesis