Anil KumarBochalya, Mahaveer Singh2017-06-032017-06-032014http://krishikosh.egranth.ac.in/handle/1/5810014673Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of stem rot of Indian mustard (Brassica juncea L.) is a necrotrophic, soil borne fungal pathogen. This pathogen initiates infection by myceliogenic and carpogenic germination of sclerotia surviving in soil. The pathogen has wide host range and difficult to control through fungicides, hence the studied were carried to find out effective rhizobacteria and non-conventional chemicals for the management of S. sclerotiorum as resistance inducers. The cultures of S. sclerotiorum were maintained and multiplied on PDA and isolation and characterization of bacterial isolates were made on King’s ‘B’ medium plates. Three species of genus Bacillus, three species of genus Rhizobium, two species of genus Pseudomonas, one species of genus Arthrobacter and Micrococcus were isolated from soil, rhizoplane and rhizosphere of mustard field. Maximum mean per cent control was recorded by Pseudomonas-1 followed by Pseudomonas-2 and Bacillus-1under screen house as well as field conditions after challenge inoculation with pathogen. Screening of non-conventional chemicals under in vitro conditions showed that salicylic acid SA) followed by acetyl salicylic acid (ASA) and indole butyric acid (IBA) inhibited maximum mycelial growth at 100 ppm concentration. Maximum mean per cent control was recorded by Bavistin at 100 ppm concentration after 6 weeks of inoculation both under screen house and field conditions followed by SA and ASA. All the rhizobacteria showed compatible reaction against all non conventional chemicals and fungicide tested at 10 and 50 ppm concentrations under in vitro conditions. Maximum per cent control was recorded by combined application of Bavistin+Pseudomonas-1 followed by SA+Pseudomonas-1, ASA+Pseudomonas-1, IBA+Pseudomonas-1, IAA+Pseudomonas-1, MgSo4+Pseudomonas-1, while minimum with ZnSo4+Pseudomonas-1. Maximum increase in total phenol, PPO, PAL, PO and chitinase activity was recorded by the application of Pseudomonas-1 followed by Pseudomonas-2 and Bacillus-1 at 3 days after rhizobacterial spray. Similarly, in case of non conventional chemicals, maximum increase in above mentioned biochemical parameters were recorded by the application of SA followed by ASA and IBA at 3 days after inoculation. At 6 days after inoculation, slight decrease in all these biochemical constituents were observed in all the treatments of rhizobacteria and non conventional chemicals both under screen as well as field conditions. Maximum increase in activity of total phenol, PPO, PAL, PO and chitinase was recorded in SA+Pseudomonas-1 followed by SA+Pseudomonas-2 and SA+Bacillus-1 at 3 days after challenge inoculation. Similar trends of results were also observed by the application of ASA, IBA, IAA, MgSo4 and ZnSo4 in combination with rhizobacterial isolates individually for the different biochemical parameters analysed.enenzymes, diseases, bacteria, acidity, planting, fungi, phenols, application methods, mustard, biological phenomenaThesis