Bhat, SumangalaSurya Prakash2021-09-252021-09-252016-10https://krishikosh.egranth.ac.in/handle/1/5810176231The present study was conducted to evaluate advanced breeding lines and mutant lines of mungbean [Vigna radiata (L). Wilczek] for powdery mildew resistance and to validate with previously reported molecular markers associated with powdery mildew resistance. The field experiment was conducted at the Main Agricultural Research Station, University of Agricultural Sciences, Dharwad during kharif 2014. The molecular experiments were conducted in the Department of Biotechnology. Phenotypic screening of advanced breeding lines and mutant lines along with the respective parental lines confirmed that parental lines BGS 9, Chinamung, DGGV 2, Selection 4, CO 7 and SML668 are susceptible to powdery mildew reaction and TARM 1 and TM-96-2 resistant to powdery mildew. Among the advanced breeding lines only BGT96-7 and BGT96-12 showed resistant reaction and were on par with TM-96-2. However, for other yield related traits such as number of cluster per plant, number pods per plant, number of seeds per pods and 100 seed weight these two lines were not superior to BGS9, the female parent involved in developing these lines. Advanced breeding lines were also genotyped using markers (CEDG259, CEDG304, MBSSR238, CEDG290, DMBSSR199 and CEDG191) already linked to powdery mildew resistant QTLs. None of the markers showed significant association with powdery mildew resistance in the advanced breeding lines used in this study. As they might carry additional QTLs imparting resistance to powdery mildew, BGT96-7 and BGT96-12 can be used as a resistance source while breeding for powdery mildew resistance.EnglishThesis