VENKATA NAIDU, G(MAJOR)SRINIVAS, MANDAHARI KRISHNA, N.V.V.RAMA KRISHNA, A2018-06-052018-06-052016-12http://krishikosh.egranth.ac.in/handle/1/5810049104ABSTRACT: Treatment of true anestrous lactating graded Murrah buffaloes (Bubalus bubalis) using estrus and ovulation hormonal protocols, Ovsynch (Group A), TRIU-B (Group-B) and Ovsynch plus TRIU-B (Group-C) and their influence on fertility during low breeding season was studied. The estrus detection rate was 80 (8/10), 100 (10/10) and 100 (10/10) per cent in Ovsynch, TRIU-B and Ovsynch plus TRIU-B protocols. There was no significant difference in mean time to onset of estrus among the three treatments. Most buffaloes in the present study showed intermediate to weak estrus signs in Ovsynch (Group A) on the contrary, the TRIU-B and Ovsynch plus TRIU-B showed intense to intermediate estrus signs in more (8/10) and (8/10) number of animals. Peak estrus synchrony with highest number of animals exhibited estrus between 48-72 hours with 6/10 in group A, 5/10 in group B and 24-48 hours in group C (7/10). The artificial insemination submission rate was 80 (8/10), 100 (10/10) and 100 (10/10) per cent in Ovsynch, TRIU-B and Ovsynch plus TRIU-B, respectively. Ovulation rate of 75.00 (6/8), 40.00 (4/10), 80.00 (8/10) per cent in Ovsynch, TRIU-B and Ovsynch plus TRIU-B protocols, respectively. The conception rate at induced estrus in the treatment groups involving Ovsynch, TRIU-B and Ovsynch plus TRIU-B in the treatment of true anestrous buffaloes were 40.00 (4/10), 20.00 (2/10) and 50.00 (3/10) per cent, respectively. However, overall conception rate was 60.00 (6/10), 40.00 (4/10) and 80.00 (8/10) per cent. During the treatment period in the control group, only 30.00 (3/10) and 20.00 (2/10) per cent anestrous buffaloes exhibited estrus and conceived, respectively.It was concluded from the present study that Ovsynch plus TRIU-B protocol obtained better estrus exhibition, Ovulation and Conception rate. So it was found to be effective in estrus response and overall conception rate in the treatment of true anestrous lactating buffaloes during low breeding season (December 2015 to March 2016).ennullThesis