Sharma, MridulaBahuguna, Chandni2020-11-202020-11-202020-09https://krishikosh.egranth.ac.in/handle/1/5810155380For economic and scientific dairy farming, dairy cattle and buffaloes should calve every year and to maintain this strategy, early pregnancy diagnosis is of utmost importance. Hence, the present research was aimed to study the protein profiling in blood and urine of pregnant and non-pregnant Sahiwal cattle for early pregnancy diagnosis. Blood and urine samples were collected from 30 Sahiwal cattle (n= 15 pregnant and n=15 non pregnant). After studying total protein, SDS PAGE and nano LC-MS a comparison was made between the data obtained from pregnant and non pregnant animals. In this study, it was found that total protein concentration of serum increased in pregnant group as compared to non pregnant group. A continuously increasing trend of protein was observed in pregnant group after day 12 (6.36 ± 0.76 g/dl) to day 22 (6.87 ± 0.39 g/dl) whereas non specific trend was found in non pregnant group. In urine, total protein concentration increased significantly (p < 0.1) in pregnant group as compared to non pregnant group from day 16 to 22. A trend of increase in concentration from day 0 to day 16 and then decrease till day 22 was observed in both groups. Maximum protein concentration was observed on day 16 (31.6 ± 0.68 mg/dl) and minimum on day 0 (23.32 ± 0.81 mg/dl) in urine of pregnant group. In SDS PAGE of serum, specific proteins of molecular weight between 29 to 43kDa and over expression of 66 kDa protein was present in pregnant group as compared to non pregnant group. In case of urine, proteins of molecular weight 43kDa and 66kDa were over expressed in pregnant cows as compared to non pregnant cows. In nano LC-MS, total 248 proteins were identified. Out of which, 129 were up regulated and 54 were down regulated. There were 17 highly up regulated proteins having more than 30 fold change. 20 up regulated proteins in nano LC-MS corresponded to the specific band present in the SDS PAGE. Highly up regulated proteins like like lactotransferrin, Golgin A4, MYRIP, PKD1 and PWWP domain containing protein MUM1 may have the potential to be used as biomarker for the pregnancy diagnosis and development of pregnancy diagnostic kit.EnglishThesis