Dahiya, G.S.Manish Kumar2023-07-182023-07-182022-07https://krishikosh.egranth.ac.in/handle/1/5810198316The investigation was carried out to study the effect of different plant growth regulators, sugars and gelling agents on in-vitro multiplication and different potting mixtures on survival rate of in-vitro raised plants of Brahmi [Bacopa monnieri (L.)]. For in-vitro multiplication plant cultures were inoculated on MS media supplemented with different plant growth regulators (BAP/Kn and IAA/NAA), carbon sources (fructose, sucrose, glucose, table sugar and maltose), gelling agens (agar-agar, agarose, gelrite, guargum and isabgol). The maximum shoots were observed on MS medium supplemented with M2 (4.6±0.67), M12 (5.0±0.04), M32 (4.9±0.06) and M19 (5.0±0.19). The maximum number of shoots (11.8±0.22) observed when MS media is supplemented with table sugar. The very good growth/rooting was observed on ½ basal media. The well rooting plantlets were tansplanted into pots containing various components of soil, sand, vermicompost, FYM, coco pit and perlite. The maximum (100%) survival were observed in four different mix including sand : soil : FYM, sand : soil: vermicompost, sand : soil : FYM : vermicompost and coco pit : vermicompost : perlite under green house conditions. Hardened plants were successfully transferred to the field conditions.EnglishThesis