Sharma, K.M.Sarode, Dipak Kacharu2019-08-202019-08-202019-08-0718383http://krishikosh.egranth.ac.in/handle/1/5810122555Cotton (Gossypium spp.) the white gold is the world’s leading natural textile fibre and also knownas "King of fibres” is one of the best gifts that nature bestowed on mankind. In the present study, twenty five cotton (GossypiumhirsutumL.)genotypes were screened bytwenty SSR primers reported to be linked to fiber quality QTLs/part of it.(Fiber length, Fiber strength and Micronaire).ElevenRAPD and six ISSR markers were also used to assess genetic diversity amongst the cotton genotypes.Eight SSR primers primersrelated with fiber length ware found to be polymorphic. One fiber length specific primer (JESPR-305) was found to be monomorphic in all genotypes. Amongstthe 251 amplicon, 226 amplicon were found to be polymorphic showing 88.88% polymorphism using nine SSR primer. Nine primers were found to be polymorphic for fiber strength scoring a total of 230 polymorphicampliconshowing 100% polymorphism. The two primer (BNL-1047 and CIR-413) specific to macronaire value reported 100 % polymorphism. An average value of polymorphic information contain (PIC)of fiber length specific 9 primer was 0.274 and 0.430for fiber strength specific SSR marker.The CIR-246 and CIR-381 primer were found to be most informative with the PIC value 0.74 and 0.78 respectively which indicates significantly diversity between all genotypes for fiber quality (Fiber length and fiber strength). Nine RAPD primers showed 86.41 % polymorphism with an average PIC value 0.410, whereas six ISSR primers resulted84.24% polymorphism with anaverage PIC value of 0.237.The combine similarity matrix based on SSR, RAPD and ISSR similarity coefficient ranged from 0.61 to 0.91 indicating the fact that large proportions of genotype were dissimilar. The present study revealed that genotypes BN-1, PH-348, and ARB-908 for fibre quality were found to be the most diverse having a broad genetic base for the concerned fibre traits.ennullThesis