Palanisamy, MValarmathi, RRaja, S; et al.TANUVAS2020-03-032020-03-032019-12http://krishikosh.egranth.ac.in/handle/1/5810144156TNV_35thVCRI_CA_Dec-2019_CCPP-10A five years old pluriparous Murrah buffalo was presented to Large Animal Gynaecology unit with the history of calved six months back and not showing any estrous signs. On clinical examination, the animal was found to be normal, free from gross genital abnormalities with good body condition score. On rectal examination the cervix was closed with flaccid mild uterine tonicity. The ultrasonographic examination revealed two multiple small follicles (MSF) in the left ovary and 8.5 mm follicle on right ovary. Follicular study was carried out at day 5, 10 and 15 which revealed a developing follicular pattern followed by atresia. Based on observations, the case was diagnosed as postpartum anovulatory anestrum-II. The animal was supplemented with mineral mixture for three weeks. Followed by fixed time artificial insemination using indigenously prepared progesterone impregnated vaginal sponge for the period of nine days with administrationof Prostaglandin on day eight. Subsequently luteinizing hormone (LH) was administered on day 11 and three artificial inseminations were done periodically and ovulation was ensured using ultrasonography. The pregnancy was confirmed on day 24th by ultrasonography. It could be concluded that progesterone treatment with fixed time artificial insemination (FTAI) along with LH would be the successful approach in postpartum anestrum buffaloes.enVeterinary ScienceOther