Sehrawat, S.K.Gaonkar, Sachin Gokuldas2020-01-272020-01-272019http://krishikosh.egranth.ac.in/handle/1/5810142135The experiment was conducted for standardization of optimum in vitro micropropagation technique in Asiatic Lilium hybrids Nashville and Eyeliner and also to evaluate genetic fidelity of in vitro cultured plantlets. Bulb scale basal segment used as explants gave good response when cultured in MS media supplemented with varying concentration of growth regulators. MS media supplemented with NAA 1 mg/l took minimum (28.07 ± 4.77) days for shoot regeneration. However, MS media supplemented with combination of BA 2mg/l and NAA 0.5 mg/l induced maximum (5.27 ± 0.27) number of shoots per explant and also highest (82.83 ± 0.70) per cent shoot regeneration. Variety Nashville was earliest to response and formed highest number of shoots during fifth week, whereas, variety Eyeliner formed highest number of shoots during sixth week. Highest (100) per cent rooting was obtained in MS media supplemented with NAA 0.5 and 1 mg/l. But, MS media supplemented with NAA 1 mg/l obtained highest (9.66 ± 1.00) number of roots per shoot and also longest (2.38 ± 0.16 cm) root length. The potting medium consisting of cocopeat was found to be the best media for hardening with (70.83) per cent survival of plantlets grown under greenhouse conditions. The genetic fidelity of plantlets was checked by using ten SSR markers. Out of these ten SSR markers only five markers namely Ivflmre-99, Ivflmre-116 and Ivflmre-138, Ivflmre-152 and Ivflmre-168 produced clear and reproducible monomorphic bands of same size and at equal distance indicating true-to-type nature of tissue cultured plants.ennullThesis