Dr. Urmila Gupta PhutelaHarmandeep Kaur2024-04-022024-04-022024Harmandeep Kaur (2024). Production of bioenzymes from organic wastes for enhancing biogas generation from paddy straw (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.https://krishikosh.egranth.ac.in/handle/1/5810207990The present study was aimed at production of bioenzymes from organic wastes for enhancing biogas generation from paddy straw. Four types of organic wastes viz. fruit waste (mosambi, unripened mango, orange, mix fruit pulp, pineapple and peach), vegetable waste (mix vegetable, potato+ pumpkin and cauliflower), leafy waste (moringa, neem and curry leaves) and lignocelluloytic waste (paddy straw, biodigested slurry, cattle dung of desi cow and cattle dung of jersey cow) were used for bioenzyme preparation. The bioenzymes produced were characterized for their pH ,total soluble solids, total sugars, reducing sugars, proteins and cellulolytic enzyme activities (endoglucanase, exoglucanase and β glucosidase). Maximum exoglucanase activity of 1.87 Uml-1 was found in cattle dung of jersey cow bioenzyme, whereas maximum endoglucanase and β glucosidase activities were observed in case of mosambi bioenzyme with 402.29 Uml-1 and 1490.50 Uml-1 respectively. Paddy straw was pretreated with 1%, 2%, 5%, and 10% concentration of different bioenzymes. Maximum reduction of 21.5% total solids, 8.31% volatile solids, 42.2% hemicellulose, 15% cellulose and 39.2% lignin was observed in paddy straw pretreated with 10% mosambi bioenzyme. The pretreated paddy straw was used for biogas production in 2-litre capacity biogas digesters and amount of biogas was measured by water displacement method. Results indicates that maximum of 20.9% enhancement in biogas production was observed in 10% mosambi bioenzyme pretreated paddy straw. Thus bioenzyme can be prepared from any organic waste and exhibits cost effective and environment friendly biological pretreatment method for enhancing biogas generation from paddy straw.EnglishThesis