Dr. P. V. PatelVANDIP CHAUHAN2017-03-062017-03-062014http://krishikosh.egranth.ac.in/handle/1/5810003910The study was carried out to a certain the prevalence, haemato–biochemical and histopathological aspects of amphistomosis in slaughtered buffaloes at Anand and Ahmedabad Districts of Gujarat for the period of twelve months from March-2013 to February-2014. The faecal samples and intestinal contents were collected in small and clean sterilized polythene bags from the buffaloes brought to the slaughter house of Anand and Ahmedabad districts and brought to the department of Parasitology and processed for standard qualitative examination. The direct, sedimentation and floatation technique were used to detect the presence and identification of amphistome eggs in the samples. Blood samples from 50 amphistomes infected as well as 50 non-infected buffaloes were taken during the anti-mortem examination for hematological analysis and to separate serum for analysis. Various serum biochemical parameters like Total protein, Albumin, Globulin, A:G ratio, Alkaline Phosphatase (AKP),Acid Phosphatase (ACP), Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Total Bilirubin along with various hematological parameters like TEC, TLC, DLC, PCV and Hb were analysed through automatic analyserenveterinary science, parasitologystudyThesis