M. L. SATYANARAYANARASHMI, S.2024-01-032024-01-032022https://krishikosh.egranth.ac.in/handle/1/5810205498The present study was pursued to evaluate the pathomorphological and biochemical changes in cyclophosphamide induced toxicity and its amelioration by Andrographispaniculata treatment in Wistar albino rats for 45 days. The study included normal control (Group I), cyclophosphamide control (Group II), Andrographis paniculata control (Group III), Andrographis paniculata pre-treatment (Group-IV), concurrent treatment (Group-V). Toxicity was induced in rats by administration of cyclophosphamide at the dose of 200 mg/kg bw as single dose and the Andrographis paniculata aqueous extract was used at the dose rate of 200 mg/kg bw. The parameters assessed following administration of cyclophosphamide and Andrographis paniculata in different groups included clinical signs, body weight, hematology parameters, biochemical parameters, antioxidant enzymes, lipid peroxidation assay, gross pathology and histopathology. There was decrease in body weight and hematology parameters and was an increase in liver enzymes, creatinine, urea and MDA values and decrease in antioxidant levels with pathological changes in liver suggesting cyclophosphamide induced hepatotoxicity and nephrotoxicity. Cyclophosphamide toxicity was characterized by necrosis and apoptosis in spleen, liver, kidney and heart with infiltration of inflammatory cells. Loss of mucosal layer in the tip of the villi and mild desquamation of mucous cells in intestine and loss of spermatogonic cells in testes suggesting cyclophosphamide induced toxicity. The concurrent Andrographis paniculata administration partially alleviated the cyclophosphamide induced toxic effects. The pre-treatment of Andrographis paniculata rendered better acquisition of overall defence status to combat immediate overload of oxidative stress caused by cyclophosphamide and hence proven that AP as herbal protectant in alleviating cyclophosphamide induced toxicity.EnglishThesis