MUTHA RAO .M (MAJOR)SRINIVAS MANDASRINIVASA PRASAD .CHRAJ KUMAR RAGALA2024-07-072024-07-072024-01https://krishikosh.egranth.ac.in/handle/1/5810211550Ultrasound guided transvaginal follicular aspiration (TVFA), also known as ovum pick up (OPU) to collect oocytes from live cows for in-vitro embryo production (IVEP) has opened up greater possibilities to exploit productive and reproductive potential of the valuable donor. The present study was aimed to compare the efficacy of ovum pick-up (OPU) and In-vitro embryo production rate between Ongole and Gir breed cows. Gir (n=6) and Ongole (n=12) cows were assigned to group 1 and group 2, respectively and all the animals were subjected to FSH pre-stimulation before OPU. The mean number of follicles available for aspiration (43.89 ± 5.38 vs 41.58 ± 5.97) did not vary between groups. Size distribution of follicles indicate that small (< 4mm) and large (>8 mm) sized follicles were lower and medium (4-8 mm) sized follicles were higher in both the groups. The mean number of follicles aspirated (34.17 ± 4.80 – group 1 vs. 27.42 ± 5.09 – group 2) and the COCs recovered (18.17 ± 5.90 – group 1 vs. 16.0 ± 3.90 – group 2) did not vary between groups. A total of 109 and 192 COCs were recovered in group 1 and 2, respectively. Of the total COCs recovered, higher percentage was of viable in group 1 (100%) than in group 2 (90.63%), but the difference was not significant (P>0.05). However, significantly lower percentage of non-viable COCs were obtained in group 1 (0%) than in group 2 (9.37%). The overall mean blastocyst development rate was non significantly higher in group 1 (52.15 %) than group 2 (35.82%). The mean number of embryos produced per cow was 11.33 ± 4.99 in group 1 and 6.50 ± 2.26 in group 2. It was concluded that the efficacy of OPU was not affected by breed as both Gir and Ongole belong to Bos indicus category with similar follicular population available at aspiration. However, higher viable oocyte yield in Gir breed eventually resulted in more number of blastocysts per OPU in this breed.EnglishThesis