Varughese, Hridya SusanChitra, M. AnandaRajasekaran, RanjaniRajalakshmi, SRaj, G DhinakarTANUVAS2019-03-182019-03-182018http://krishikosh.egranth.ac.in/handle/1/5810098877TNV_IJAR_2018_1-5The attempt was made to study the response of the dog having skin infection with various underlying causes to killed S.pseudintermedius antigen. PBMC were separated and sensitized with killed S. pseudintermedius for 24 hours. cDNA was made from extracted RNA and quantitative PCR were carried out for 8 cytokines with GAPDH as housekeeping gene. IL-6 was the cytokine which was expressed in a statistically significant high level in PBMC of healthy animals to killed antigen than PBMC of infected animals. Except for the IL-6, all other cytokines were expressed at high level in pyoderma animals than healthy control, demodicosis and allergy cases. In the present study, IL-1β, IL-8 and TNF-α were the cytokines that were up-regulated and, IL-6 and IFN-γ were down-regulated in demodicosis dogs with pyoderma at apparently significant level than the other tested cytokines. IL-4 was the only cytokine that was expressed in measurable quantity in allergic cases when compared to other cytokines. Thus, it was concluded that dogs with staphylococcal pyoderma infections developed a Th1/Th2 response to fight the infection.enVeterinary ScienceArticle