Dr. Ajithkumar S.LAKSHMI MOHANAN2024-05-242024-05-242022-11-07https://krishikosh.egranth.ac.in/handle/1/5810209424Bovine theileriosis is an economically important tick- borne haemoparasiticdisease of cattle. A study on oriental theileriosis was conducted in the Department ofVeterinary Clinical Medicine Ethics and Jurisprudence, College of Veterinary andAnimal Sciences, Mannuthy during the period from May 2021 to April 2022. Onehundred and fifty nine cows in different farms of Thrissur and Wayanad districts werescreened using blood smear examination. Out of 159 samples screened, 74 (46.54 percent) animals were positive for theileriosis. The PCR was conducted in 16 clinicalcases positive for theileriosis by blood smear, using genus and species specificprimers for T. orientalis. The genus specific primers amplified 1098 bp fragment andspecies specific primers amplified 601 bp fragment of T. orientalis in all 16 cases. Real time PCR assay was developed using recombinant plasmids of parasite (p23) andhost (hprt) to estimate the level of parasitemia. Major clinical signs noted were dullness, reduced milk production, excessive salivation, anorexia and pallor of mucous membranes. Haematological study of thediseased animals revealed anaemia with low erythrocyte count, haemoglobin andvolume of packed red cells. A significant increase was noted in total leukocyte countand monocyte levels in infected animals. On serum biochemistry, creatinine valuesrevealed a significant reduction in diseased animals. No significant difference wasnoted in the values of malondialdehyde (MDA) and reduced glutathione, betweendiseased and control animals. Sixteen cows positive for theileriosis on blood smearexamination were classified into two groups. Animals belonging to group I weretreated with a single dose of Inj. Buparvaquone @ 2.5 mg/kg body weightintramuscularly. In addition to buparvaquone, group II animals were supplementedwith a single dose of Inj. vitamin. E –Se combination @ 1 mL/45 kg intramuscularly. Haematological parameters of animals of group I were improved significantly aftertreatment, but no significant difference was noted in group II animals. In group I, asignificant increase was noted in total protein and A:G ratio after treatment, whereasonly total protein revealed a significant increase in animals of group II. In animals ofgroup I, significant reduction was noted MDA levels following treatment, whereas no significant difference was found with reduced glutathione. Significant reduction was noted in MDA and reduced glutathione revealed significant increase in group IIanimals. Osmotic fragility test revealed that erythrocytes of cattle infected with T. orientalis were more susceptible to haemolysis and the fragility of erythrocytes decreased in both groups after treatment. Parasitemia levels did not reveal any significant difference in both groups after treatment. Statistical analysis between groups showed no significant difference in any of the parameters after treatment.EnglishThesis