THAKUR, MANISHASHARMA, SONAM2016-12-262016-12-26201347890http://krishikosh.egranth.ac.in/handle/1/92850ABSTRACT The present investigation aims at ‘In vitro selection of ginger (Zingiber officinale Rosc.) against Fusarium oxysporum f.sp. zingiberi’. Callus was obtained from root explants procured from in vitro cultures of ginger cv. Himgiri. MS medium supplemented with 1.0 mg/l BA and 0.5 mg/l 2, 4-D proved to be the best medium for callus induction. Shoot differentiation and regeneration from callus was achieved on MS medium supplemented with 1.0 mg/l BA and 0.1 mg/l NAA. Well-developed multiple shoots formed roots on the same medium after 2-3 passages of subculture, thus eliminating the necessary step of in vitro rooting. Cell line selection against Fusarium was done by using culture filtrate of Fusarium oxysporum f.sp. zingiberi as a selective agent. Calli was selected at 15 per cent level of fungal culture filtrate and multiplied on the previously standardized callus induction medium. Then the selected calli were cultured on shoot differentiation medium. After shoot differentiation the rooted controls as well as selected plantlets were tested through in vitro testing using mycelium suspension of Fusarium oxysporum f.sp. zingiberi. One resistant plant could be obtained after in vitro selection which showed slight symptoms of Fusarium infection which was hardened by transferring to sterilized potting mixture.enThesis