Dr. Jinesh Kumar N.S.ANJU MARY JOHN2024-01-272024-01-272022-12-29https://krishikosh.egranth.ac.in/handle/1/5810206574The study was conducted in six clinical cases of dogs presented with corneal wounds to Teaching Veterinary Clinical Complex, Pookode. Canine amniotic membranes were collected during elective caesarean section from healthy dogs. Amniotic membrane was peeled off from the foetal sac and was rinsed with sterile saline solution containing 0.1 mg/ml gentamicin and 2.5 mcg/ml amphotericin B. The membranes were then flattened on to nitrocellulose paper and were stored in 98 per cent glycerol at room temperature. Corneal wound healing was assessed in all the dogs after grafting with the fresh preserved canine amniotic membrane.Preoperatively, the animals were given moxifloxacin eye drops, one drop four times daily and atropine eye drops, one drop once daily. Under general anaesthesia, debridement and scarification of cornea was done using sterile cotton buds and lance blade. Three layers of canine amniotic membrane were secured in place by suturing over bulbar conjunctiva at 360° in five dogs and on cornea itself in one dog, using 8.0 vicryl as suture material. This was followed by a third eyelid flap. Topical instillation of antibiotic eyedrops was continued until healing while atropine eye drops were continued for four days postoperatively. Third eyelid flap suture was removed on 10th postoperative day. Postoperatively all the animals were evaluated on 10th, 20th, 30th and 45th days. Values of Schirmer tear test, abnormal ocular discharge and number of blinks per minute were higher in initial days, which later got reduced post-operatively. FDT was positive for all the six dogs on presentation, which became negative in five dogs on 10th day and in another dog on 20th day, suggestive of complete re- epithelialisation of cornea. A progressive reduction in corneal oedema, and improvement in corneal clarity was observed. The superficial corneal vascularisation reduced significantly and only regressing vessels noticed in three animals on 45th day of observation. All the animals regained corneal transparency, except for haziness in one dog. Corneal pigmentation was not observed in four cases, whereas two dogs showed increased extent of pigmentation at the nasal limbus. The dogs with corneal pigmentation were Chinese Pugs who already had corneal pigmentation at the initial presentation. Positive neuro-ophthalmic tests and normal vision were present in all the animals on 45th postoperative day.EnglishThesis