Sharma, AnshuYadav, Vandna2017-08-192017-08-192006http://krishikosh.egranth.ac.in/handle/1/5810029122The present investigation was carried out on 15 randomly selected milch buffaloes divided into three groups on the basis of lactation of an organized farm, to study the foot and mouth disease virus type specific antibodies in milk and serum following FMD vaccination. Milk and serum samples collected before vaccination i.e. 0 day and on 7, 14, 28, 42 and 56 days post vaccination, were analyzed for the detection of FMD virus type specific antibodies by liquid phase blocking ELISA (LPBE) and virus specific IgG1, IgG2 and IgA antibody response by indirect double antibody sandwich ELISA. In all the cases milk samples were treated with Arkalone, centrifuged and top fat layer was removed before conducting the assay. Significant FMD virus type specific antibody titres (LPBE, IgG1, IgG2 and IgA) were detected in milk and serum of buffaloes on different days post vaccination, though the levels of antibodies were lower in milk as compared to serum. FMD virus type specific IgG1 was found to be the predominant subclass as compared to IgG2 and IgA both in milk and serum of vaccinated buffaloes. Milk and serum LPBE, IgG1, IgG2 and IgA antibody titres were positively correlated with values of regression coefficient (R) as 0.6376, 0.506, 0.434 and 0.396, respectively. Milk LPBE antibody titres against different FMD virus types were significantly different showing higher antibody titres against FMD virus type O followed by A and Asia 1. Serum LPBE antibody titres against FMD virus type O and Asia 1 were not significantly different, except for type A. Milk IgG1 and IgG2 antibody titres against FMD virus type O, A and Asia 1 were not significantly different. The serum IgG1, IgG2 and IgA antibody titres against FMD virus type O were significantly low as compared to type A and Asia 1 in all the three lactations of buffaloes. Inspite of the limitation of milk for detection of antibodies against FMD virus types that it can only be used in milch animals, we can not undermine the importance of present study. In country like India milch animals are more important and kept in large number than draught animals.enThesis