SINGH, K.N.PRIYA, SHIVAM2021-09-082021-09-082007-01-06https://krishikosh.egranth.ac.in/handle/1/5810175344THESISTo develop an efficient tissue culture protocol for callus induction and regeneration in two popular indica cultivars i.e. IR 64 and Swarna was undertaken. The explants used for initiation of culture were mature seeds. For callus induction in Swarna and IR 64, MS (Murashige and Skoog, 1962) media was used with different concentration of 2, 4-D, Kinetin, L-proline and Casine hydrolysate in 10 combinations. Best callus induction in terms of friability and growth in Swarna were obtained in the medium having combinations of MS media supplemented with 2, 4-D (2 mg/ l) + Kinetin (0.4 mg/ l) + L-proline (500 mg/ l) + Casein hydrolysate (300 mg/ l) + maltose (30 g/ l) + agar-agar (0.8 % w/v) with 87 % callus induction percentage, whereas in IR-64, it was found best with MS media supplemented with 2, 4-D (2 mg/ l) + L-proline (500 mg/ l) + Casein hydrolysate (300 mg/l) + maltose (30 g/ l) + agar-agar (0.8 % w/v) with 78 % callus induction percentage.EnglishThesis