Koshy JohnSHIJU SHAJI2020-09-072020-09-072018https://krishikosh.egranth.ac.in/handle/1/5810150897Leptospirosis has been identified as re-emerged potentially zoonotic disease with wide distribution in developing countries like India. In regions like Wayanad, leptospirosis is underdiagnosed and prevalent serovars are unknown due to lack of previous studies and due to lack of facilities for proper diagnosis. In this scenario, a comprehensive study was conducted for identification of prevalent serovars and detection of infection in domestic animals of Wayanad district by molecular methods. Total 71 samples including blood, serum, urine and tissue samples were collected from clinically suspected cases of leptospirosis and from different regions of Wayanad district. All the samples collected were subjected to total DNA extraction and PCR was carried out using G1/G2 and lipL32 based primers. The test could detect amplicons in positive control but no positive results were yielded by clinical samples.Out of total 62 serum samples tested by MAT, the standard serological test for leptospirosis, 20 samples have given positive result. The most predominant serovar identified was Pyrogenes which was followed by Grippotyphosa, Bataviae, Icterohaemorrhageae, Javanica, Pomona, Australis and Canicola. Mixed infections were also observed which included Pyrogenes and Bataviae, Pyrogenes and Javanica, Grippotyphosa and Canicola, Pyrogenes and Australis, Grippotyphosa and Javanica. The MAT titre of agglutinating serovars varied from 1:3200 to 1: 200. Highest MAT titre observed was 1:3200 which was against serovar Pyrogenes.enThesis