Puvarajan, BLurthu Reetha, TSelvaraj, JManickam, RTANUVAS2020-03-092020-03-092020-02http://krishikosh.egranth.ac.in/handle/1/5810145077TNV_20thMVC_PP_Feb-2020_PA36Clostridium novyi type B causes infectious necrotic hepatitis (INH) in sheep and less frequently in other species of livestock. A 7 year old Gir cow after postmortem examination revealed icterus with large amount of serosanguineous fluid in the abdominal cavity and the liver samples were received for bacteriological examination and was subjected to anerobic cultivation followed by biochemical characterization in association with Grams staining which was identified as Clostridium novyi. Further the liver tissue was subjected to PCR and were found positive for C. novyi type B alpha toxin genes but negative for Clostridium haemolyticum andother clostridia. Histologically, there was coagulative necrosis surrounded by a rim of inflammatory cells and large numbers of gram-positive rods and concluded as a definitive diagnosis of infectious necrotic hepatitis (INH).In ruminants, also known as black disease, INH has rarely been reported in cattle . An amplicon of 427 bp corresponding to 16SrRNA gene sequence was obtained and this PCR Assay was standardized as this was confirmed simultaneously with the type culture DNA extract (ATCC 25758). Moreover owing to the Clostridial infections in cattle causing acute death, this is the first report of identifying the Clostridium novyi through molecular means of earlier detection paving way as an efficient tool for precise diagnosis of Clostridium novyi type B infections in cattle.enVeterinary SciencePresentation