Parthiban, M.Jaiganesh, R.Aruni, A. WilsonMeignanalakshmi, S.Ramadass, P.TANUVAS2019-12-302019-12-302007http://krishikosh.egranth.ac.in/handle/1/5810138993TNV_AV_77(3)_2007_265-270The rapid isolation of M. bovis was attempted in THP-l cells (Macrophage cell line). THP-l cells infected with M. bovis showed characteristic cytopathic effect 48 hr after infection. The bacteria had grown very well in the first passage itself, without any blind passages in cell culture. The presence of bacteria in the cell culture fluid was confirmed by Ziehl-Neelsen staining and nested PCR amplification of insertion sequences.en-USVeterinary ScienceAnimal BiotechnologyArticle