Khangia, BipinNarzary, Jonaki2022-02-092022-02-092017-07https://krishikosh.egranth.ac.in/handle/1/5810181925An experiment was conducted under shade net house at the experimental farm, Department of Horticulture, Assam Agricultural University, Jorhat, during October 2016 to May 2017 to study “Efficacy of biofertilizers and bioregulators in macropropagation of banana”. The experiment was laid out in Randomised Block Design with fourteen treatments comprising of two cultivars viz., V1 (Cheni Champa) and V2 (Kachkal) and seven different substrates consisting of T0 (Sawdust), T1 [Sawdust+ VAM (30 g)], T2 [Sawdust+ Trichoderma viride (30 g)], T3 [Sawdust + VAM (30 g)+ Trichoderma viride (30 g)], T4 [Sawdust+ IBA (2500 ppm)+ Azospirillum (30 g)], T5 [Sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)] and T6 [Sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)] replicated three times. The results revealed that maximum growth and quality planting materials could be achieved by incorporation of additives like biofertilizers and bioregulators to the sawdust substrate. Among the treatments, V1T6 [Cheni Champa with sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)] had significant influence on time taken for bud initiation (23.22 days), number of secondary suckers (4.78), tertiary suckers (17.33) and percentage of suckers (90.33 %). It was observed that with the advancement of the hardening period, the growth characters of tertiary suckers increased from the initial stage to the end of the hardening. In terms of growth parameters of tertiary suckers, sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g) showed better result in both the cultivars. V2T6 [Kachkal with sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)] recorded significant influences on plant height (29.07 cm), stem girth (6.91 cm) and leaf length (35.63 cm) but V1T6 [Cheni Champa with sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)] gave best results for leaf breadth (15.33 cm), and weight of tertiary suckers (219.98 g), number of roots (19.56), root girth (1.45 cm) and the highest survival percentage (99.78 %). However, V1T4 [Cheni Champa with sawdust+ IBA (2500 ppm)+ Azospirillum (30 g)] recorded the highest number of secondary roots (84.89), total chlorophyll content (1.53 mg/g FW) and leaf relative water content (73.95 %). Economics of production revealed the highest B:C of 1.41 under V1T6 [Cheni Champa with sawdust+ BAP (4 ml)+ Bacillus subtilis (30 g)+ VAM (30 g)]. Thus, application of biofertilizers and bioregulators exhibited positive impact on macropropagation of banana.EnglishThesis