Karthikeyan, K.Muniasamy, S.SankarGanesh, D.Achiraman, S.Kumar, V. Ramesh SaravanaArchunan, G.TANUVAS2018-08-282018-08-282013-03http://krishikosh.egranth.ac.in/handle/1/5810069871Chemo-signals are among the reliable non-invasive methods for estrus detection in mammals. Water buffalo is a silent heat animal and, hence, there is search for chemo-signals which would be effective non-invasive indicators of estrus state. We analyzed the faecal chemical cues during the estrous cycle in buffalo and to find the estrus-specific faecal volatile compounds adopting bull behavior assay. The faecal samples were collected at three phases of the estrous cycle (i.e., proestrus, estrus and postestrus) and subjected to gas chromatography–mass spectrometry analyses. We found 27 volatile compounds in the faeces of buffaloes, of which 4-methyl phenol (4mp) and trans-verbenol (tv) were found only in estrus faeces.en-USVeterinary ScienceLivestock Production and ManagementArticle