PATHOLOGICAL AND MOLECULAR DIAGNOSIS OF LYMPHOPROLIFERATIVE DISEASES (LPDS) IN CHICKEN
Loading...
Date
2021
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A total of 155 lymphoproliferative diseases (LPDs) suspected cases from the chicken farms (n=27) were studied. Highest incidence of LPDs (20 farms out of the 27 farms) were recorded in the Namakkal region. The higher and lower incidence of LPDs was noticed in the flocks with age group between 46-55 wks and 86 to 95 wks respectively. The highest incidence of MD, LL, RE, concurrent infection of MD and LL, concurrent infection of MD and RE, and concurrent infection of MD, LL and RE was recorded in the age group between 36 to 45 wks, 46 to 55 wks, 20 wks and 26 wks, 46 to 55 wks, 20 wks and 25 wks and 26 to 35 wks respectively. Polymerase chain reaction revealed the highest incidence of MDV (63.87 per cent), followed by concurrent infection of MDV and LL (22.58 per cent), LL (7.10 per cent), concurrent infection of MDV, LL and REV (2.58 per cent), REV and concurrent infection of MDV and REV (1.29 per cent).
Grossly, enlargement, mottling, greyish spots/foci with nodules in the visceral organs were noticed in LPDs affected birds. Histopathologically, MD affected organs like liver, spleen, kidney, heart, lungs, proventriculus, gizzard, small intestine, mesentery, pancreas, skin, comb, skeletal muscle, bone marrow, brain, sciatic nerve, eye, ovary and thymus showed PLC infiltrations with loss of architecture, replacement, displacement and atrophic changes of constituent structures with score of 1 to 5. In addition to PLC infiltrations, the liver showed mitotic figures in lymphoid cells and occasional MD cells; medial hypertrophy of
artery, lymphocytolysis and mild reticulum cell hyperplasia in the spleen; glomerulitis, intertubular oedema, crystals and eosinophilic cast in the tubules of the kidney; intravascular lymphoid cells in the lung; vasculitis and mild hyperkeratosis in the comb; congestion, focal satellitosis, gliosis, axonal spheroids, perivascular cuffing, perivascular oedema and neuronal vacuolation in the brain; Perivascular PLC infiltration in the choroid, iris and ciliary body with subcortical vacuolation of lens fibres in the eye and intravascular PLC infiltration in the ovarian stroma were also noticed.