GREEN SYNTHESIS OF SILVER NANOPARTICLES, THEIR CHARACTERIZATION AND ANTIMICROBIAL POTENTIAL
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Date
2017
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Department of Molecular & Cellular Engineering, Jacob Institute of Biotechnology & Bioengineering, Sam Higginbottom University of Agriculture, Technology & Sciences Allahabad- 211007, U.P. (India) 2017
Abstract
Nanotechnology is currently a flourishing field provides a novel way to fabricate nanoparticles exploring biological sources. The study focuses on the green synthesis of silver nanoparticles using plant extracts as reducing, capping and stabilizing agents. Aqueous silver ions when exposed to plant leaf extract were reduced and resulted in a colour change indicating the formation of silver nanoparticles. The silver nanoparticles were successfully synthesized under variable process parameters using P. niruri extract. The synthesis of silver nanoparticles was accomplished using different plant parts S. cumini. Abiotic stress induced in vitro Z. mays plants showed progressive enhancement in biochemical estimation as well as in the green synthesis of silver nanoparticles. The synthesized silver nanoparticles were characterized by UV-Vis spectrophotometer, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Fourier Transform Infra-red Spectroscopy (FTIR) techniques. The UV-Vis spectra showed peak at 420 nm. SEM and TEM revealed that the synthesized silver nanoparticles are spherical and circular in morphology and the overall size is between 4 to 50 nm. FTIR spectroscopy analysis showed the presence of flavonoid, polyphenols and amide groups likely to be responsible for the green synthesis of silver nanoparticles. The synthesized nanoparticles showed efficient antimicrobial activity as well as free radical scavenging activity. The studies describing the biosynthesis of silver nanoparticles followed by the investigation of antimicrobial and scavenging activities may be useful for green nanotechnology research opening a new arena in this field.
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Ph. d. thesis
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