ISOLATION AND IDENTIFICATION OF POTENTIAL PROBIOTIC BACTERIA FROM THE GUT OF SELECTED FRESHWATER ORNAMENTAL FISHES 3065
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Date
2020-06
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JAU, JUNAGADH
Abstract
he study was conducted to isolate and identify probiotic bacteria from the gut of
Xiphophorous helleri and Betta splendens. For isolation of gut microbes five fishes of each
variety were collected. Eleven bacterial strains were isolated from the freshwater ornamental
fishes. The eleven group represented by isolates of C1, C2, C4, C9, C11, C13(a), C13(b),
C14, C19, FSB and FSS were selected for biochemical tests. Bacteria isolated from the gut of
Xiphophorous helleri were identified as Enterobacter, Enterococcus faecalis and Bacillus
subtilis by observing their colony morphology, physiological and as well as some
biochemical characteristics. Microscopically they were Gram-negetive, irregular or round
shaped, non- motile and few bacterial strains were catalase negative. Bacillus cereus was
isolated from Betta splendens. It was Gram-positive and catalase-positive. From this study, it
was concluded that the eleven bacteria isolated from intestine could be potentially developed
for their use as probiotic bacteria in fish feeds.