“Evaluation of Microbial Consortia for Plant Growth Promotion and Management of Fusarium Wilt of Chickpea”
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Date
2022-09-09
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Banda University of Agriculture and Technology, Banda
Abstract
Chickpea (Cicer arietinum L.) is one of the most important pulse crop of Bundelkhand region
of Uttar Pradesh. Wilt disease caused by Fusarium oxysporum f. sp. ciceris is one of the major
constraints in production of chickpea in the regions and causes 10% annual yield loss (Singh
and Dahiya, 1973). The microbial consortium is a group of species of microorganisms that act
together as a community and synergistic way. Indigenous isolates of bacterial bio-agents viz;
Pseudomonas fluorescens, Bacillus subtilis and Mesorhizobium ciceri were isolated from root
nodules and rhizospheric field collected chickpea field of Crop research farm of the University
and identified on the basis of morphological characters and biochemical tests. Potential isolates
of fungal antagonists such as T. harzianum and T. viride were collected from Plant Pathology
Laboratory of BUAT, Banda. Indigenous fungal and bacterial bio-agents were tested in vitro
for their efficacy against pathogen. Among Six native fungal antagonists T. harzianum (TH-2)
was found highly effective against the pathogen, which inhibited maximum growth of the
pathogen 67.03% followed by T. harzianum (TH-3) which inhibited 66.85% mycelia growth of
pathogen. Among three bacterial isolates, P. fluorescens showed maximum inhibition growth
of pathogen (63.98%) followed by B. subtilis (48.70%) and M. ciceri (36.93%). The
compatibility of potential bio-agents was also tested through dual culture technique. All the
bacterial bio-agents found compatible with each other and the fungal antagonist T.
harzianum (TH-3) found potential against pathogen and compatible with all the bacterial bioagents. The bio-agents which found effective and compatible with each other were selected for
development of consortia. Laboratory studies were conducted to study the effect of pH (4.5-
9.0) and temperature (20-400C) on colony growth of pathogen and bio-agents. In study of the
effect of pH the fungal antagonist T. harzianum was attained maximum growth (80 mm) at pH
6.0 whereas the pathogen (Foc) and P. fluorescens attained highest growth 72.50 mm and
56.80 mm respectively at pH 6.5. While B. subtilis and M. ciceri both grew best at neutral pH 7
with 75.50 mm and 18.5 mm respectively. In study of the effect of temperature on the growth
of microbes, the pathogen (Foc), T. harzianum and M. ciceri exhibited maximum growth 65.33
mm, 80 mm and 37.83 mm respectively at 280C, while B. subtilis and P. fluorescens exhibited
maximum growth 76.67 mm and 58.17 mm respectively at 32°C. A pot culture experiment was
conducted under net house condition during Rabi season 2021-22 with 14 treatments and three
replications in CRD to evaluate the efficacy of microbial consortia for plant growth promotion
and management of chickpea wilt. The results revealed that the treatment (T13) in which seed
treatment with T. harzianum + B. subtilis + P. fluorescens + M. ciceri found most effective
which enhanced maximum root and shoot length 40.10 and 48.72 cm respectively and that
treatment also provided 100% seed germination maximum reduction (75.11%) in wilt
incidence.
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Citation
Girijesh Kumar Jaisval. 2022 “Evaluation of Microbial Consortia for Plant Growth Promotion and Management of Fusarium Wilt of Chickpea” Thesis, M.Sc.