STANDARDIZATION OF MICROPROPAGATION PROTOCOL FOR MASS PRODUCTION OF DRAGON FRUIT (Hylocereus undatus (Haw.) BRITTON AND ROSE)

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dc.contributor.advisorGUNNAIAH, RAGHAVENDRA.
dc.contributor.advisorPATIL, S. N.
dc.contributor.advisorG. PRABHULING
dc.contributor.advisorD. P. PRAKASHA
dc.contributor.advisorCHITTAPUR, REKHA.
dc.contributor.authorCHONGLOI, LHINGNEIVAH.
dc.date.accessioned2024-05-24T16:42:37Z
dc.date.available2024-05-24T16:42:37Z
dc.date.issued2022-04
dc.description.abstractDragon fruit (Hylocereus undatus (Haw.) Britton and Rose) or pitaya is an emerging tropical and subtropical fruit crop belonging to Cactaceae. Due to its attractive colour, high nutritional composition and luscious taste, commercial cultivation has been picking up in India over the past decade. However, the supply of planting material is a bottleneck in meeting the increasing demand. The conventional propagation method through stem cutting is time-intensive due to its slow growth and seeds doesn‟t assure true to type planting material. The upsurge requirement for planting material demands tissue culture generated planting material, which ensures the crop's clonal fidelity and availability of planting material throughout the year. Therefore, we investigated the standardisation of the micropropagation protocol of dragon fruit in this study. Different types of explants (in vitro stem, in vitro leaf and in vivo stem) were inoculated onto Murashige and Skoog's (MS) medium supplemented with different concentrations of 2,4-D and BAP for their shoot proliferation. A 100.00 % response to shooting induction, the maximum number of shoots per explant (9.94) and longest shoot length (2.26 cm) were recorded when in vivo stem (E3) explant was cultured on MS0 + 1.5 ppm BAP. The in vitro stem explant (E1) cultured on MS0 + 2.0 ppm BAP exhibited the earliest bud break (12.00 days). The in vitro proliferated shoots were further rooted in MS media supplemented with different concentrations of NAA and IBA. Among the treatments, cent per cent rooting with the least number of days for root initiation (6.00 days) and the longest root per shoot (5.24 cm) was recorded in the treatment MS0 + 0.1 ppm IBA. Average roots per shoot (5.71) were highest in MS0 + 0.5 ppm NAA. The well-developed shoots and roots were transferred to protrays filled with a different potting mixture of sand, soil and vermicompost alone or in combination. Sand + vermicompost (1:1) (v/v) exhibited the maximum survival (100.00 %), the maximum number of shoots/plantlet (7.66), longest mean shoot length (10.61 cm), the maximum number of roots per plantlet (11.27) and longest mean root length (10.38 cm). The genetic fidelity of the in vitro regenerated plants was assessed by using eight ISSR markers on 25 randomly selected seedlings and four sets of mother plants with their respective regenerants. The per cent polymorphism was highest (57.69 %) in seedlings and lowest (1.92 %) in M3 and M4 populations. Principal coordinate analysis and dendrogram constructed through neighbour-joining indicated greater genetic diversity in the seedlings. High genetic proximity was observed among tissue cultured plantlets with their respective mother plants. The economic analysis revealed that the cost of production of planting materials through tissue culture was higher by Rs. 5.09 as compared to that of the conventional method of stem cuttings.
dc.identifier.otherUHS18PGD269
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810209458
dc.keywordsHorticulture
dc.keywordsFruit Science
dc.keywordsFruits
dc.keywordsDragon Fruit
dc.keywordsBritton
dc.keywordsRose
dc.keywordsPitaya
dc.keywordsTropical
dc.keywordsSubtropical
dc.keywordsCactaceae
dc.keywordsNutritional
dc.keywordsPropagation
dc.keywordsClonal
dc.keywordsFidelity
dc.keywordsMicropropagation
dc.keywordsProtocol
dc.keywordsIn Vitro
dc.keywordsInoculation
dc.keywordsProliferation
dc.keywordsTreatments
dc.keywordsVermicompost
dc.keywordsShoots
dc.keywordsPlantlet
dc.keywordsSeedlings
dc.keywordsRegenerants
dc.keywordsPolymorphism
dc.keywordsDendrogram
dc.keywordsUHS18PGD269
dc.keywordsT774
dc.language.isoEnglish
dc.pages199p. (Due to pdf. conversion there is a difference in pages of soft copy and hard copy).
dc.publisherUniversity of Horticultural Sciences, Bagalkot. (College of Horticulture, Bagalkot).
dc.subFruit Science
dc.themeSTANDARDIZATION OF MICROPROPAGATION PROTOCOL FOR MASS PRODUCTION OF DRAGON FRUIT (Hylocereus undatus (Haw.) BRITTON AND ROSE)
dc.these.typePh.D
dc.titleSTANDARDIZATION OF MICROPROPAGATION PROTOCOL FOR MASS PRODUCTION OF DRAGON FRUIT (Hylocereus undatus (Haw.) BRITTON AND ROSE)
dc.typeThesis
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