Characterization of seed sources of sissoo (Dalbergia sissoo roxb.) using molecular markers
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Date
2015
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Publisher
CCSHAU
Abstract
The ecological and economic importance of Dalbergia sissoo and declination of its
population in its natural habitat promoted the present study. Based on desired economically
important characters, seed sources were selected from nine provenances covering different
agro-ecological regions of Haryana. The progeny testing was done for one year in the nursery
of Forestry department, CCS H.A.U., Hisar. Genetic diversity was evaluated by using RAPD
markers. Significant variations were observed among the provenances as well as agroecological regions for all the plus tree characters viz., total height, diameter at breast height,
clear bole height, crown spread and straightness. Correlation among most of the characters
was also significant with maximum value 0.923 between total height and diameter at breast
height. Variations among morphological characters of pods and seeds were significant. High
heritability with high genetic advance was observed for pod and seed weight character. The
maximum genotypic and phenotypic correlation (0.598 and 0.519) was observed between
seeds per pod and 100 pod weight, and seed length and 100 seed weight, respectively. Ample
genetic variation was also observed for germination percentage, seedling height, collar
diameter, number of branches per seedling, height of first branch, root length, fresh weight
and dry weight among the progenies of the selected plus trees. Fresh weight and dry weight
characters were found highly significantly correlated, followed by root length and total height.
Among all the progenies, the progeny of Ambala seed sources at a stage of six month and
Hisar progenies at 12 month age was found outstanding. Genetic diversity among the plus tree
progenies assessed with the help of 60 RAPD primers displayed the values of genetic
similarity coefficient ranging from 0.49 to 0.81, which indicated a broad genetic base of
Dalbergia sissoo in selected area of Haryana state.
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Keywords
Dalbergia, Genetic diversity, Provenance, Plus tree