STUDIES ON EFFECTS OF SYSTEMIC HORMONAL AND INTRAUTERINE HERBAL THERAPY ON POST-PARTUM UTERINE INVOLUTION, OVARIAN REBOUND AND SERVICE PERIOD IN HOLSTEIN FRIESIAN COWS
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Date
2008
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AAU, Anand
Abstract
The present research project "Studies on effects of systemic hormonal and intrauterine herbal therapy on post-partum uterine involution, ovarian rebound and service period in Holstein Friesian cows." was undertalcen on 28 Holstein Friesian cows. The objectives of the study were to observe the effect of Prostaglandin and oxytocin injections on uterine involution and resumption of ovarian activity during post-partum period, blood plasma metabolic concentration. Progesterone (P4) hormone concentration on post-partum and the effect of herbal antibacterial preparation (intrauterine) on uterine involution and resumption of ovarian activity, induction of oestrus and conception rate during post partum period. The animals were divided into four groups each group having seven animals. The four groups were: Group I:- Control ; Group II:-Vantab, Three intra-uterine infusions given at weekly interval postpartum. Group III:- Oxytocin, 50 units and PGF2a, 25 mg (Iliren) I/M. immediately after parturition and Group IV:- PGF2a, 25 mg (Iliren) 1/M, immediately after parturition . Blood samples were collected from parturition (0 day) to 75th day at fortnightly interval through jugular venupuncture for estimation of biochemical constituents by auto analyser (Selectra Junior) and progesterone hormone estimation by Radio Immuno Assay (RIA) technique. The HF cows under the study revealed complete regression of pregnancy CL by mean interval of 8.50 ± 0.81 (range 3-21) days. There was non significant difference (P<0.05) found all groups of trait. In Group-Ill and Group-IV pregnancy CL lysis occurred in short time as compared to Group-I, Group-II. The mean interval from calving to first post-partum oestrus was 31.65 ± 1.45 (range 23 to 45) days in HF cows under study. Highly significant difference (P < 0.05) was observed between groups for first post-partum oestrus, being higher in control group of animals as compared to treatment groups. The mean period of uterine involution was found to be 29.71 ± 0.71 (range 22- 37) days. The mean period of uterine involution was 32.57 ± 1.25, 31.71 ± 0.91, 25.85 ± 1.10 and 28.71 ± 0.91 days respectively for group I, II, III and IV. There were highly significant difference (P<0.01) for period of in uterine involution between various treatment groups. Plasma glucose concentrations in cows under the present experiment were 57.79 ± 0.50 (range 50 to 67) mg %. The Difference within treatment groups was highly significant (P < 0.01). The values for glucose increased 60 days post-partum. Plasma total protein concentration in cows under experiment was 7.25 ± 0.05 (range 6.65 to 7.87) g/dl There no significant difference (P<0.05) between treatment mean of different groups for plasma total protein concentration but there was a significant different (P<0.05) between period mean. Plasma cholesterol concentration in cows under the study was 106.92 ± 2.53 (range 63.20 to 158.02) mg/dl. Differences within treatment and between groups were highly significant (P < 0.01). Plasma Triglycerides concentration in cows under study was 12.76 ± 0.14 (range 8.3 to 17) mg/dl. Differences within treatment and between groups were highly significant (P < 0.01). The average plasma concentration of progesterone in the cattle was 2.09 ± 0.13 (range 0.1 to 7.00) ng/ml through out post partum period. There were non significant difference (P<0.05) between treatment mean but there was a significant different (P<0.05) between period mean. The overall mean values of service periods were 122.39 ± 6.90 (range 62- more than 150) days. In the present study, it was observed that interval from calving to first service postpartum was non significant in all the groups. The mean values of service periods were 142.28 ± 21.53, 123± 9.28, 109 ± 9.05, 114.85 ± 11.02 respectively in group 1,11, III and IV. The treatments given may enhance the fertility in post-partum HF cows. The animals under Group-Ill and Group-IV had faster involution and shorter service period as compared to animals treatment group-ll and control group-I.
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Veterinary Obstetrics and Gynaecology, A Study