Studies on the spermatological properties and standardization of cryopreservation protocol for cobia, Rachycentron canadum

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dc.contributor.advisorDr. C. Judith Betsy
dc.contributor.authorMs.Sweety Singh
dc.contributor.otherDr. J. Stephen Sampath Kumar
dc.contributor.otherDr. Cheryl Antony
dc.date.accessioned2022-08-10T05:29:52Z
dc.date.available2022-08-10T05:29:52Z
dc.date.issued2021
dc.description.abstractMilt was collected from Rachycentron canadum brooders reared in open sea cages of Marine Finfish Hatchery Project, Rajiv Gandhi Centre for Aquaculture, Muttom, Kanyakumari district. Spermatological parameters like sperm density, motility duration, motility pattern and motility score were observed in the collected milt. After recording the initial parameters of raw milt, the milt was cryopreserved using 0.85% Physiological Saline and Marine Fish Ringer’s Solution with Dimethyl Sulphoxide (DMSO) as cryoprotectant (10% and 5% level in diluents) at three dilution ratios such as 1:40, 1:80 and 1:120. The diluted milt was equilibrated for 10 min at 4oC in the cold handling unit and rapid frozen for 10 mins by liquid nitrogen vapour freezing method. The cryopreserved spermatozoa were analysed once in 10 days for 30 days after thawing of the straws at 30° C for 30 s. The thawed milt was observed under microscope for the same parameters of the pre-frozen milt and the data were recorded. Raw milt of R. canadum exhibited the highest post-thaw motility duration of 58 s whereas the motility score was 9 and all the spermatozoa showed forward and circular movement. The sperm density in the milt of R. canadum spermatozoa was 3.04×109 Cells/ml, 2.51×109 Cells/ml and 1.96×109 Cells/ml at 1:40, 1:80 and 1:120 dilution ratios respectively. R. canadum milt diluted with 0.85% Physiological Saline showed the highest post thaw motility duration (55±1 s) with a motility score of 9. There were 89% of live cells with forward and circular motility pattern, whereas the post-thaw motility parameters are low in Marine Fish Ringer’s solution. The motility duration was 34.33±1.52 s, motility score was 2 with 74% live cells and weak vibratory motility pattern. The values of motility duration were statistically significant between the two extenders used (p˂0.001). R. canadum milt cryopreserved with 10% DMSO exhibited the highest post-thaw motility duration of 55±1 s, whereas the sperm motility duration was decreased in 5% DMSO with motility duration of 34.33±1.52 s. When DMSO was used at 5 and 10% concentration for cryopreservation of R. canadum milt, the difference in motility pattern observed was only due to the extender used. Spermatozoa exhibited only forward and circular movement throughout the experiment when DMSO was used at 5 and 10% concentration along with PS, whereas the motility pattern was weak vibratory when DMSO was combined with MFRS. No change in motility score was noticed when R. canadum milt was cryopreserved with DMSO, however, the score was influenced by the diluent used and the cryopreservation period. The values of motility duration were statistically significant between the two concentrations of DMSO (p˂0.05). R. canadum milt diluted at 1:40 showed the highest post-thaw motility duration of 55±1 s as compare to 1:80 (53.66 s) and 1:120 (50.33 s) dilution ratios. It can be inferred that the motility pattern and motility score of spermatozoa diluted at 1:40, 1:80 and 1:120 changed depending on the duration of cryopreservation and type of extender used. The present study revealed that Physiological Saline with 10% DMSO at 1:40 dilution ratio is suitable for cryopreservation of R. canadum spermatozoa.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810186154
dc.language.isoTamilen_US
dc.pages123en_US
dc.publisherFisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thoothukudien_US
dc.subAquacultureen_US
dc.themeStudies on the spermatological properties and standardization of cryopreservation protocol for cobia, Rachycentron canadumen_US
dc.these.typeM.F.Scen_US
dc.titleStudies on the spermatological properties and standardization of cryopreservation protocol for cobia, Rachycentron canadumen_US
dc.typeThesisen_US
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